May 10, 2022
Source: Clin Cancer Res. 2022 May 10;clincanres.4413.2021. doi: 10.1158/1078-0432.CCR-21-4413. Online ahead of print.
Authors: Jitka Fucikova, Michal Hensler, Lenka Kasikova, Tereza Lanickova, Josef Pasulka, Jana Rakova, Jana Drozenova, Tessa Fredriksen, Marek Hraska, Tereza Hrnciarova, Klara Sochorova, Daniela Rozkova, Ludek Sojka, Pavel Dundr, Jan Laco, Tomas Brtnicky, Ivan Praznovec, Michael J. Halaska, Lukas Rob, Ales Ryska, An Coosemans, Ignace Vergote, David Cibula, Jirina Bartunkova, Jerome Galon, Lorenzo Galluzzi, Radek Spisek.
Purpose: The successful implementation of immune checkpoint inhibitors (ICIs) in the clinical management of various solid tumors has raised considerable expectations for patients with epithelial ovarian carcinoma (EOC). However, EOC is poorly responsive to ICIs due to immunological features including limited tumor mutational burden (TMB) and poor lymphocytic infiltration. An autologous dendritic cell (DC)-based vaccine (DCVAC) has recently been shown to be safe and to significantly improve progression-free survival (PFS) in a randomized Phase II clinical trial enrolling patients with EOC (SOV01, NCT02107937).
Experimental design: We harnessed sequencing, flow cytometry, multispectral immunofluorescence microscopy, immunohistochemistry to analyze (pre-treatment) tumor and (pre-treatment and post-treatment) peripheral blood samples from 82 patients enrolled in SOV01, with the aim of identifying immunological biomarkers that would improve the clinical management of patients with EOC treated with DCVAC.
Results: Although higher-than-median TMB and abundant CD8+ T cell infiltration were associated with superior clinical benefits in patients with EOC receiving standard-of-care chemotherapy, the same did not hold true in women receiving DCVAC. Conversely, superior clinical responses to DCVAC were observed in patients with lower-than-median TMB and scarce CD8+ T cell infiltration. Such responses were accompanied by signs of improved effector functions and tumor-specific cytotoxicity in the peripheral blood.
Conclusions: Our findings suggest that while patients with highly infiltrated, "hot" EOCs benefit from chemotherapy, women with "cold" EOCs may instead require DC-based vaccination to jumpstart clinically relevant anticancer immune responses.